A 36-year-old Wyndham Vale woman was taken to hospital with life-threatening injuries following a collision on Citylink in Burnley on the morning of Friday, May 9.
Emergency services responded to reports of a car, that police believe the woman was driving, travelling against the flow of traffic before colliding with a truck about 2.10am.
The truck driver, a 51-year-old Aintree man, was not injured.
Police said the investigation into the circumstances surrounding the collision remains ongoing.
Anyone who witnessed the incident or has dashcam/CCTV footage from the area prior to the collision is urged to contact Crime Stoppers on 1800 333 000 or crimestoppersvic.com.au